life diary

life diary

Tuesday, May.3,2016,fine and warm.

Treat English as an daily language

I was waken on 7:40 as usual, then I pick up my telephone and open up the Shanbei APP to remember and revise some words. After going to the washroom, I washed my hair and face. Then I put on my trousers and go to “YaXiange”—a small restaurant which provide usa delicious food but precious food. It is just OK for me to eat the food here, I am not the thrifty man anymore, because of the health is at the first place. In the restaurant, I ate some Gnocchi and an egg for the breakfast. Although it was a little bit salty, but I really liked it, it made me feel comfortable. Several students had came to the laboratory before I got to the laboratory. I’m not that sure about what would happen in the morning. I think no matter what happen, what I should do first is to finish my English words—it is my necessity in my each daily life. I should hold on doing it until my English skills go up on a good stage. Today’s words were difficult to remember, so it took me about another half a hour to finish the task. And time flied to 9 o’clock. There was one more important thing to do that to help my tutor. It was also a tough thing to do with. Because it was my first time to finish it from start to the end by myself. The most difficult part of it is how to provide a good and original idea which had really racked my brain. At first, I had always thought that I could suggest some good ideas, but things were not that easy, and things tended to be wrong at the end. It seemed that nothing can be suitable to the initial thought. The only things that I could do was to remedy the particle. During the writing, a question occur to my mind that why can’t I to study English well enough, what’s wrong with my methods on learning English, although I had always stick on it for a long time. So I lost in thought and tried to find some good answers to it. Finally, I found a good answer to it that is to use English at anytime and everywhere. From my comprehension on this explanation is treat English as a daily language, so that we can get more closer to it.

美文:人生起起落落 中英文

【英语佳句】 ups and downs of life 人生起起落落 When life gets hard and you want to give up, remember that life is full of ups and downs, and without the downs, the ups would mean nothing. 当生活很艰难,你想要放弃的时候,请记住,生活充满了起起落落,如果没有低谷,那站在高处也失去了意义。

Life has its ups and downs. You will never fully appreciate the ups unless you have some downs. 人生充满了起起落落,没有在低处历练过的人,也无法享受高处的快感。

Life is full of ups and downs. The tirck is to enjoy the ups and have courage during the downs. 人生充满了起起落落,关键在于:在顶端时要好好享受,在低谷时不失勇气。 Life is like a see-saw ride, full of ups and downs …… But when you are down, a good friend will jump on the other seat tp bring you back up again.

人生就像骑跷跷板,时高时低。当你处于低处时,你的好朋友会跳上另一头,把你重新带回高处。 Life is full of ups and downs. If you want to truly enyoy life, you should take the rough with the smooth. 人生充满了起起落落,若要真正享受人生就应该能接受人生的沉浮。

mean的短语有哪些

mean的短语有哪些 mean表表示…的意思; 意思是的意思,那么你知道mean 的短语有哪些吗?接下来为大家整理mean的短语搭配,希望对你有帮助哦! I mean 1. 我想,我说,我的意思是(用于澄清或纠正,或用于提出理由、解释) 我想说,我真的没有财产。 I mean, it's not as if I owned property. mean business 1. 认真,热心 mean to say 1. 意思是说;想说 你是不是想说你有新的发现?。 do you mean to say you've uncovered something new?. mean well 1. 用意是好的,初衷是好的 mean streets 1. 贫民街区;治安混乱的城区

南布朗克斯的贫民街区。 the mean streets of the South Bronx. no mean ; 1. 很好的,很棒的 这是一大成就。 it was no mean feat. mean business【非正式用语】 1. 急切,迫切 by all means 1. 当然;当然可以 by any means 1. 用尽各种可能的办法;无论如何 无论如何也不是一个容易的对手 not by any means an easy opponent. by means of 1. 使用;由于 他们靠耐心和牺牲而取得了成功 They succeeded by means of patience and sacrifice. by no means 1. 并没有;绝不 这次讲话绝不能等闲视之 This remark by no means should be taken lightly.

人生礼颂

A Psalm of Life 人生礼颂 [有声] Herry Wadsworth Longfellow/享利.沃兹渥斯.朗费罗 Tell me not in mournful numbers, 请别用哀伤的诗句对我讲; Life is but an empty dream! 人生呵,无非是虚梦一场! For the soul is dead that slumbers 因为沉睡的灵魂如死一般, And things are not what they seem. 事物的表里并不一样。 Life is real! Life is earnest! 人生是实在的!人生是热烈的! And the grave is not its goal; 人生的目标决不是坟墓; Dust thou art , to dust returnest, 你是尘土,应归于尘土。 Was not spoken of the soul. 此话指的并不是我们的精神。 Not enjoyment , and not sorrow, 我们的归宿并不是快乐, Is our destined and our way; 也不是悲伤, But to act, 实干 That much to-morrow. 才是我们的道路, Find us farther than to-day. 每天不断前进,蒸蒸蒸日上。 Art is long , and time is fleeting. 光阴易逝,而艺海无涯, And our hearts , though stout and brave. 我们的心哪——虽然勇敢坚强,Still , like muffled drums , are beating 却像被布蒙住的铜鼓, Funeral marches to the grave。 常把殡葬的哀乐擂响。 In the world’s broad field of battle, 在这人生的宿营地, In the bivouac of Life, 在这辽阔的世界战场, Be not like dumb,driven cattle! 别做无言的牲畜任人驱赶, Be a hero in the strife! 做一名英雄汉立马横枪! Trust no future.howe’er pleasant! 别相信未来,哪怕未来多么欢乐! Let the dead Past bury its dead! 让死去的往昔将死亡一切埋葬!

attitude to life 人生态度

attitude to life 人生态度 We have a saying "destiny" character creation, therefore, attitude, character and personality is attitude is destiny, so if the right attitude fate, fate nature also went toward the direction of "correct" development, it is logical, but in the life "attitude" how to "set"? But what is more complex, more likely, it is not so simple, or just take a single options can finish. Because of "life", the life span 70 to estimate assume a paragraph, such as 10 years before and after 10 years of life needs and ideological attitude is not the same growth to 20 years old, he again into 30 acceptable and the environment is not the same, and so on, has grown to 70 years old, affirmation is living in another world, so that each to a stage, it must be different degree of growth, and learn the different environment, needs and responsibility or said. Also means different stages, will have different life "attitude", will make the necessary adjustments "attitude", to face your own health life. Basically should cultivate themselves, keep the following principles can: 我们有句话说“性格创造命运”,因此,态度就=性格,性格又=命运,所以态度就=命运,如果态度正确了,命运自然也就朝往“正确”的方向发展了,这是必然的逻辑但在人生的“态度”上要如何“设定”?可就比较复杂些,更可能,就并不是那么简单了,或只采取单一选项就能完成。因为“人生”全长的寿命,假设预估70岁为一个全程段落,譬如10岁前以及10岁后,的生活需求与思想态度就不一样了成长到20岁再进入30岁,他所接受的环境肯定又不一样了,以此类推,一直成长到70岁,肯定又是生存在另一个世界中,所以说每到一个阶段,都有它一定不同程度的成长,与学习的不同环境而有所变化,或说需求与责任。也就是说不同的阶段,就要具备不同的人生“态度”,就要做出必要的调整“态度”,来健康面对自己的人生。 基本上应该培养自己,时时保持以下几个原则即可: 1. With an open mind 2 the attitude to learn 3 good interpersonal interaction relationship 4 maintain healthy body 5. Have a happy family 6 work or business can be stable development 7. To see the future prospects 1.开阔的心胸与视野 2.积极学习的态度来充实内在 3.建立良好人际互动的关系 4.保持健康的体魄

The story of life人生的故事

The story of life. Sometimes people come into your life and you know right away that they were meant to be there, to serve some sort of purpose, teach you a lesson, or to help you figure out who you are or who you want to become. You never know who these people maybe (possibly your roommate, neighbor, coworker, long lost friend, lover, or even a complete stranger), but when you lock eyes with them, you know at that very moment they will affect your life in some profound way. And sometimes things happen to you that may seem horrible, painful. and unfair at first, but in reflection you find that without overcoming those obstacles you would have never realized your potential, strength, willpower, or heart. Everything happens for a reason. Nothing happens by chance or by means of good luck. Illness, injury, love, lost moments of true greatness and sheer stupidity all occur to test the limits of your soul. Without these small tests whatever they may be, life would be like a smoothly paved, straight, flat road to nowhere. It would be safe and comfortable, but dull and utterly pointless. The people you meet who affect your life, and the success and downfalls you experience, help to create who you are and who you become. Even the bad experiences can be learned from. In fact, they are probably the most poignantand important ones. If someone hurts you, betrays you, or breaks your heart, forgive them, for they have helped you learn about trust and the importance of being cautious when you open your heart. If someone loves you, love them back unconditionally, not only because they love you, but because in a way, they are teaching you to love and how to open your heart and eyes to things. Make every day count. Appreciate every moment and take from those moments everything that you possibly can for you may never be able to experience it again. Talk to people that you have never talked to before, and actually listen. Let yourself fall in love, break free, and set your sights high. Hold your head up because you have every right to. Tell yourself you are a great individual and believe in yourself, for if you don't believe in yourself, it will be hard for others to believe in you. You can make of your life anything you wish. Create your own life and then go out and live it with absolutely no regrets. Most importantly, if you LOVE someone, tell him or her, for you never know what tomorrow may have in store. And learn a lesson in life each day that you live. That's the story of life.

高通量测序及分析

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仁爱七下Unit-5-Our-School-Life短语及句子练习

Unit 5 Our School Life短语及句子 Topic 1: 1. 在学校大门口______________________ 2. 去/ 来上学 ______________________ 3. 骑自行车__________________________ 4.乘地铁 __________________________ 5. 乘公共汽车________________________ 6. 乘飞机 _________________________ 7. 步行____________________________ 8. 坐小汽车 _________________________ 9. 乘火车___________________________ 10. 坐船 ___________________________ 11. 走路上学________________________ 12. 看电视 _________________________ 13. 做家庭作业______________________ 14. 看电影 _________________________ 15. 上一节英语课_____________________ 16.放学后____________下课后 _______ 开始上课________________________ 17.在工作日,平日 ___________________ 18. 到家____________________________ 19.上床睡觉 _______________________ 20. 一会儿__________________________ 21. 见朋友 ________________________ 22. 踢足球__________________________ 23. 打篮球 _________________________ 24.去游泳__________________________ 25. 去钓鱼 _________________________ 26.去公园/ 动物园_______________________ 27.听音乐 ____________________

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短语归纳 look after \ take care of 照顾on the first day 在第一天 Class 1,Grade 7 7年级1班play football 踢足球 after school 放学后be\come from 来自 be good at \do well in 擅长fly kites 放风筝 go home 回家listen to music 听音乐 play a game 玩游戏wear glasses 戴眼镜 at school 在学校all the lessons 所有的课程 talk about 谈论over there 那里 a lot of hobbies 许多爱好 用法集萃 love\like doing sth 喜欢做某事let’s +动词原形让我们 I am\My name is 我叫welcome to +地点欢迎来到 This is 这是be good at \do well in doing 擅长做 in Class…Grade…在几年级几班live with…in…和谁住在哪里 I’m …year old. 我几岁了。I have…hair.我留着……头发 典句背诵 What’s your name?你叫什么名字? Nice to meet you! 很高兴见到你。 I love reading. 我喜欢阅读 Now let’s meet our new classmates. 现在让我们认识下我们的新同学。 I often play football after school.放学后我经常踢足球。 She is tall and slim. 她个头很高,身材苗条。 He is from Nanjing. 他来自南京。 He is good at Maths. 他擅长于数学。 Millie is 11 years old.米莉11岁。 They are all very nice.他们都很好。 I am good at dancing. 我擅长于跳舞。 语法 连系动词be 的一般现在时 动词be 的三变化am is are. 我(I)用am ,你(you)用are ,is 用于他(he)她(she)它(it),单数名词用is,复数名词都用are 句型结构: 1、肯定句:主语+am\is\are …It is a football. 2、否定句:主语+am\is\are+not…It is not a football. 3、一般疑问句:Am\Is\Are+主语+其他? Is it a football? 回答Yes, it is. No, it isn’t 4、特殊疑问句:疑问句+am/is/are +主语+其他?What’s your name? Unit 2 Let's play sports! 短语归纳 play sports 做运动many times a day 一天许多次 play football/tennis 踢足球/打网球talk about 谈论 after school 放学后go swimming 去游泳 a member of …中的一员come from 来自

英语阅读:Life Is to Be Whole 人生在于完整

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下一代测序技术 摘要:DNA测序技术对生物学的发展有着最根本的意义。Sanger法测序经过了30年的应用和发展,而在过去三年中,以454, solexa, SOLiD为代表的高通量测序平台已经大幅度降低了测序成本,提高了测序速度,成为基因组测序市场的主流。在此基础上,各种下一代测序技术正在快速研发,将使基因组测序和重测序的通量和成本更加平民化,为基因组学、遗传学、生物医学和健康科学等领域的发展创造更加广阔的前景。本文将对所有新的测序技术的原理、优势和应用进行总结和展望。 1977年Maxim、Gilbert发明的化学降解法测序技术和Sanger发明的双脱氧末端终止法测序技术不仅为他们赢得了诺贝尔奖,也使得从DNA序列层面研究分子遗传学成为可能。特别是后者,从最开始的凝胶电泳到越来越高通量的毛细管电泳,从开始的手工操作到越来越多自动测序仪的出现,各种改进的Sanger 测序技术统治了DNA测序领域三十年,至今仍在长片段测序,大片段文库测序方面有广泛的应用。人类基因组计划(HGP)的完成就是靠Sanger测序法。 在耗费了庞大成本的人类基因组计划宣布完成之后,越来越多的物种基因组测序工作对测序成本和通量提出了更高的要求,新一代测序技术(也被称为第二代测序技术)开始登上历史舞台。2005年454 life science公司率先推出了焦磷酸测序技术,使测序成本较Sanger法降低了100倍,速度快了(提高)100倍,人类基因组测序逐步进入了100,000美元时代。如今,454 FLX测序仪(Roche Applied Science)、基于“边合成边测序”的Solexa测序仪(Illumina Inc.)和使用“边连接边测序”的SOLiD测序仪(Applied Biosystems)已经成为基因组测序市场的主流机型。除此之外,2008年一年内又有HeliScope单分子测序仪(Helicos)和Polonator(Dover/Harvard)两种测序机型商品化。 在NHGRI(美国人类基因组研究中心)的支持和推动下,未来几年内测序成本将在目前基础上再下降100倍,最终使个人基因组测序成本降至1000美元,人类将革命性的进入个人基因组时代。高通量和低成本的测序技术将进入到普通实验室,基因组测序的简单化将使分子生物学飞跃发展,个人基因组测序产业化也将对健康医学等领域产生革命性的影响。本文将首先对目前已经商品化的新一代测序技术(454、Solexa、SOLiD、HeliScope)做一介绍和比较,再对正在研发中的各种下一代测序方法(第三代测序技术)的原理和应用做一详细的介绍和展望。 1. Roche 454测序技术 2005年454生命科学公司在《自然》杂志发表论文,介绍了一种区别于传统Sanger法的全新高通量测序方法,将测序成本降低了100倍以上,开创了第二代测序技术的先河,454测序仪也成为最先商品化的第二代测序仪。正是在此基础上,其它如Solexa、SOLiD等第二代测序仪才相继问世。454测序技术的原理在于首先使用乳液PCR(emulsion PCR)技术(图一a)扩增已经连接上接头的基因组文库片段,扩增子结合在28 μm的磁珠表面,将乳液破坏后用变性剂处理磁珠,再将含有扩增子的磁珠富集到芯片表面,用测序引物进行测序。在测序过程中,454使用了一种“焦磷酸测序技术”(Pyrosequencing),即在合成DNA 互补链的过程中,每加入一种单核苷酸(dNTP),如与模板链配对结合,就会释放出一个焦磷酸,与底物腺苷-5’-磷酸硫酸(APS)在A TP硫酸化酶作用下合成A TP,与荧光素(Luciferin)一起在荧光素酶(Luciferase)的作用下,会发出一个光信号,由芯片背后连接的电荷耦合装置(CCD,Charge Coupled Device)捕捉。454测序技术合成DNA链使用的是普通单核苷酸,没有任何标记,合成中也没有切割基团等生化反应,因此读长可以达到300-400bp。但没有阻断(block)和去阻断(de-block)过程也意味着对连续重复单核苷酸的阅读只能根据信号强度来判断,容易对其中插入和缺失碱基阅读错误。454测序技术相比较其他第二代测序技术如Solexa和SOLiD, 在读长上有着巨大的优势,但是目前成本要略高。总体而言,高读长使得454技术比较利于De Novo拼接和测序。

超完整英语励志名言短语大合集

英语培训https://www.360docs.net/doc/424676185.html, 超完整英语励志名言短语大合集 1、While there is life there is hope. 一息若存,希望不灭。 2、I am a slow walker,but I never walk backwards. ( America) 我走得很慢,但是我从来不会后退。(亚伯拉罕.林肯美国) 3、Never underestimate your power to change yourself! 永远不要低估你改变自我的能力! 4、Nothing is impossible! 没有什么不可能! 5、Nothing for nothing. 不费力气,一无所得。 6、The man who has made up his mind to win will never say "impossible ". (Bonaparte Napoleon ,French emperor ) 凡是决心取得胜利的人是从来不说“不可能的”。( 法国皇帝拿破仑. B.) 7、I will greet this day with love in my heart. 我要用全身心的爱来迎接今天 8、Do what you say,say what you do 做你说过的,说你能做的 9、I can make it through the rain. I can stand up once again on my own. 我可以穿越云雨,也可以东山再起 10、All things come to those who wait. 苍天不负有心人

My outlook on life(我的人生观)

My outlook on life As the old saying goes that “character determines destiny ”, a different attitude to life may make an adverse end. If you feel life is lacking in the power which you need and the motivation you require, it’s time to transform your point of view. I always deem that pleasant things are not impracticable. Nevertheless does you suffer from how hard an setback is, you are ought to face life with a full smile. Once you lose heart to life, how will god be in favor of you? A too powerful pessimism is a prison from which a man must escape from if he is to enjoy the life to full. You have to adapt yourself to society. So transform your concern into trust, and learn to surpass the uncertainties and doubt. Do not fritter away energy and time on endless complaining. In stead, use them to do some significant things. Whether you are sixteen or sixty, you should keep a curious heart that will never be prostrate, a native desire to learn about everything, and the joy to pursue a booming career. C ome on! Work like you don’t need money. Live like you have never been heart. When you treat your life positively and make it your daily discipline, you will find happiness goes along.

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关于生活体验等(常考,特别重要)★★★ 1. 产品科技含量technological element of product 2. 大学生创业university students’ innovative undertaking 3. 双赢局面win-win situation 4. 首创精神pioneering spirit 5. 振兴中华make China powerful and strong; 6. 富裕affluence 7. 繁荣boom 8. 购买力buying powe r 9. 竞争competition 10. 生活费用cost of living 11. 解决问题crack the nuts 12. 大家公认it is universally acknowledged that 13. 全力以赴bring one’s talent into full play 14. 适应新的形势变化adapt/adjust/accommodate oneself to new environment/change 15. 获得成功achieve/accomplish success 16. 提出观点advance/put forward/come up with arguments/ideas 17. 较好地驾驭生活be a better pilot of one’s life 18. 对……很好的了解have a better understanding of 19. 把某种因素考虑进去take sth. into account/consideration; 20. give much thought to 21. 品味人生/自由/青春savor the life/freedom/youth 22. 交流经验share experience/ideas/problems/knowledge 23. 发挥,起到积极作用play an important/active/great role/part 24. 面临困难危险be confronted with danger/difficulty; in the face of 25. 阻碍了成功stand in the way of success; be an obstacle/barrier to 26. 缩小差别narrow/fill the gap/gulf (between city and country) 27. 把成功/错误归因于attribute/owe one’s success/failure to 28. 对……重要be vital/important/indispensable to 29. 施加压力exert/put pressure on 30. 重视attach/assign much importance/significance to 31. 把注意力集中在focus/concentrate one’s attention/efforts upon 32. 抓住机会grab/seize/take the opportunity 33. 有可能there is (little/much) possibility/likelihood that 34. 献身于devote/dedicate/commit oneself to a cause/career 35. 真正重要的是what really matters/counts is 36. 影响思想,态度shape one’s thinking/attitude 37. 实现自己的理想,愿望realize/fulfill/achieve one’s dream/hope; 38. 减轻压力,紧张reduce/alleviate the stress/pressure 39. 提高社会地位upgrade social status; enhance social position /advance/enhance the 41. 随着生活节奏的加快with the quickening pace of modern life 42. 充满激情have a burning desire for

Life is a journey人生如旅

Life is a journey人生如旅 From the hell to the heaven, There's no straight way to walk. Sometimes up, sometimes down. Hope creates a heaven for us, Despair makes a hell for us. 地狱天堂路遥遥, 理想捷径无处寻。 起起落落前行路, 一线希望造天堂, 千般绝望坠地狱。 Some choices are waiting for me, Which one on earth is better? No God in the world can help me, Choosing is the 1)byname of freedom, Different choice makes different future. 前行歧路须选择, 究竟哪条会更好? 绝无上帝可依凭, 自由别名乃选择, 不同抉择造异境。 It's stupid to put eyes on others. I have to make up my own mind, Going my way to the destination. 盲随他人不明智, 吾须定夺前行路, 不达目标誓不休。 Facing success or failure, It's no need to care too much. Only if I've tried my best, It’s enough for my simple life. 纵然面对成与败, 谨记无须太在意。 只要吾已竭全力, 淡然此生无所系。 Life is a journey人生如旅 From the hell to the heaven, There's no straight way to walk. Sometimes up, sometimes down. Hope creates a heaven for us, Despair makes a hell for us. 地狱天堂路遥遥, 理想捷径无处寻。 起起落落前行路, 一线希望造天堂, 千般绝望坠地狱。 Some choices are waiting for me, Which one on earth is better? No God in the world can help me, Choosing is the 1)byname of freedom, Different choice makes different future. 前行歧路须选择, 究竟哪条会更好? 绝无上帝可依凭, 自由别名乃选择, 不同抉择造异境。 It's stupid to put eyes on others. I have to make up my own mind, Going my way to the destination. 盲随他人不明智, 吾须定夺前行路, 不达目标誓不休。 Facing success or failure, It's no need to care too much. Only if I've tried my best, It’s enough for my simple life. 纵然面对成与败, 谨记无须太在意。 只要吾已竭全力, 淡然此生无所系。

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