超声波辅助提取石阡苔茶多糖工艺的优化

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超声波辅助提取石阡苔茶多糖工艺的优化

作者：陈仕学王岚代鸣王一帆杨芳

来源：《湖北农业科学》2014年第15期

摘要：为了研究超声波辅助提取石阡苔茶[Camellia sinensis （L.） O. Ktze.]多糖的最佳提取工艺，采用单因素和正交试验进行研究，得到了提取石阡苔茶多糖的最佳工艺条件为乙醇体积分数40%，超声功率100 W，浸提时间30 min，料液比1∶40 （g∶mL）。此条件下多糖的平均提取率为4.92%，RSD为0.42%。可见，超声波辅助提取法缩短了浸提时间，节约了材料，提高了石阡苔茶多糖的提取率。

关键词：苔茶[Camellia sinensis （L.） O. Ktze.]；多糖；超声波辅助法；提取率；工艺优化

中图分类号： TQ461 文献标识码：A 文章编号：0439-8114（2014）15-3609-04

Optimizing the Polysaccharide Extraction Process from Shiqian Moss Tea by Ultrasonic-assisted Method

CHEN Shi-xue，WANG Lan，DAI Ming，WANG Yi-fan，YANG Fang

（Bioscience and Chemistry Department， Tongren University/Key Laboratory of Special Animal and Plant Resource in Fanjing Mountain

/The Search Center of the Conservation and Utilization of Wildlife Resources in Fanjing Mountain，

Tongren 554300， Guizhou， China）

Abstract：The optimal extraction process of polysaccharide from Shiqian Moss Tea[Camellia sinensis （L.） O. Ktze.] was studied with the ultrasonic-assisted method. The single factor and the orthogonal test were adopted. The results showed that its optimal conditions were 40% ethanol， 100 W ultrasonic power， 30 minutes ultrasonic digestion time and 1∶40（g∶mL） solid-liquid ratio. Under these conditions，the avarage extraction rate of the polysaccharide was 4.92% and that of RSD was 0.42%. The ultrasonic-assisted method can greatly shorten the extraction time， save the materials and enhance the extraction rate of Shiqian Moss Tea’s polysaccharides.

Key words： Moss tea[Camellia sinensis（L.） O. Ktze.]；polysaccharides； ultrasonic-assisted method； extraction rate； process optimization

收稿日期：2014-01-20