Notch1胞内段在鼻咽癌组织及鼻咽癌细胞株中的表达研究

Notch1胞内段在鼻咽癌组织及鼻咽癌细胞株中的表达研究张芳婷;万汇涓;李明华;刘晓翌;尹美珺n;叶静;于洁

【期刊名称】《中国全科医学》

【年(卷),期】2012(015)024

【摘要】Objectives To investigate the expression of Notchl intracellular domain ( NIC ) in non - kertain nasopharyngeal carcinoma ( NK - NPC ). Methods RT - PCR and immunocytochemical staining were used to determine 52 biopsy specimens and different cell - lines of NPC. Results By RT - PCR, NIC genes were expressed in different NK - NPC cell lines, but the expression of high - differentiated CNE1 cell line was most remarkable. The results of immunocytochemical staining performed correspondingly were similar to it. By the results of immunocytochemical staining of NPC specimens, NIC expression was higher in NK - NPC than in chronic nasopharyngitis ( P = 0. 005 ), closely correlated with differentiation ( P < 0. 05 ), but not with gender or age ( P >0. 05 ). In undifferentiated NK - NPC, there was scarcely positive expression but more lymphocyte positive expression. In differentiated NK - NPC, positive expression was obvious and cell membrance, penplasm and nucleus were seen. Conclusion Notch signaling participates in occurance of non - keratin naspharyngeal carcinoma, and Notchl expression is positively correlated with differentiated degree of NK - NPC.%目的探讨Notch1胞内段(NIC)在非角