填料选择指南

CONTENTS
Sephadex LH-20 1 YMC 2 Fuji 3 Daiso 4 MERCK MERCK Fractogel 1 2 3 AMBERCHROMTM CG MCI GEL Hisep 1 2 3 8 10 11 12 13 14 16 17 18 19 23 25 27 31 AFC AFC AFC HIC HIC HIC 33 36 38 41 42 44 45 46 48 49 49 49 50 53 54 56
1 GE Healthcare 2 TOSOH 3 Cellufine IEC 1 GE Healthcare 2 TOSOH 3 Cellufine AFC 1 GE Healthcare 2 TOSOH 3 Cellufine HIC 1 GE Healthcare 2 TOSOH 3 Cellufine
1 2 1 2 3 Millipore 1 Millipore Vantage L 2 Millipore QuikScale 3 Milliopore IsoPak 1 QuikSep 2 Prep30

Cellufine
Cellufine
Cellufine
2

C18 Sephadex LH-20
YMC
Merck
Fuji
Daiso Toyopearl HW Toyopearl IEC Toyopearl AFC Toyopearl HIC
MCI GEL Amerbchrom CG HiSep RP Sephadex G Sephadex LH-20 Sepharose SEC Superdex Sephadex IEC Sepharose IEC Sepharose AFC
3

Sephadex LH-20
Sephadex LH-20
Sephadex LH-20
Sephadex Sephadex G-10 G-25 Sephadex G Sephardex LH-20 : : : : LH-60
Sephadex LH-20
300mg
/ml
Dimethyl sulphoxide Pyridine Water Dimethylformamide Saline Methanol Methane dichloride Chloroform 1 Propanol Ethanol2 Isobutanol Formamide Methylene dichloride Butanol Isopropanol Tetrahydrofuran Dioxane Acetone Acetonitrile3 Carbon tetrachloride Benzene Ethyl acetate Toluene
1 2 3 1% 1% 2.5mL/g
mL 4.4 4.2 4.0 3.8 3.9 3.8 3.8 3.8 3.7 3.6 3.6 3.6 3.6 3.5 3.3 3.3 3.2 2.4 2.2 1.8 1.6 1.6 1.5
/g 4.6 4.4 4.4 4.2 4.1 4.1 4.1 4.1 4.0 3.9 3.9 3.9 3.9 3.8 3.6 3.6 3.5 2.6 2.4 2.2 2.0 1.8 1.6 pH
4-5KD 20% 1% 18-111um 70um 27-163 um 103 um 720cm/min 60 cm/min 2-11 2-13 pH2 121 4 4-25 4-8 pH6-8 20% 0.04% 20 40
17-0090-10 17-0090-01 17-0090-02 Sephadex LH-20
25g 100g 500g
4

Sephadex LH-20
Phyllanthus niruri
17 18
Sephadex LH-20
Sephadex LH-20
HIV-1
Sephadex LH-20
1 2 13
Sephadex LH-20 LH-20
26 3,4 5 11 12 24
Sephadex
Sephadex LH-20 Sephadex LH-20
5

Sephadex LH-20
Sephadex LH-20
1993 FDA Sevag Sephadex LH-20 Sephadex LH-20
OH Sephadex LH-20 DEAE
8 10 27 11
AH-1 AH-2 DHP-1 DHP-2
Sephadex G100 AG-2
Sephadex G200
6

Sephadex LH-20
Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20
1
Sephadex LH-20
2 3 4 5 6 7 8 9 10 11 12 13 14 15
Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Linalooj.geraniol Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20 Sephadex LH-20
16 17 18 19 20 21 22 23 24 25 26 27
repandusinic acid A 27-
phyllanthus nirurri
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27
, , , , , , , , , , ,
1988:16(11):17 1994 25 7 344-346 1994 29 6 449 1994 29 12 1989 20 11 1986 11 934 7
9 554-555 1995 313 4 188 188
1995 21 1990 21 4
1995 319-320 1994 36 11 895 1996 21 5 290-292 1996 161 1996 1996 232 283
, Ogata,T.,et al. Amersham Pharmacia Biotech Dr Hans Henke Dr Hans Henke Dr Hans Henke Dr Hans Henke Dr Hans Henke Dr Hans Henke , ,
2001 32 2
107-108 1996 283
Aids-Res-Hum-Retroviruses,November,Vol.8,11(1992)1937-1944 Sephades LH-20 Data File 18-1107-22,High performance chromatograpby of steroids, terpenoids and low molecular weight peptides Preparative Gel Chromatography on Sephadex LH-20,Huthig Cerlag Heidelgerg, 1995:123 Preparative Gel Chromatography on Sephadex LH-20,Huthig Cerlag Heidelgerg, 1995:193 Preparative Gel Chromatography on Sephadex LH-20,Huthig Cerlag Heidelgerg, 1995:195 Preparative Gel Chromatography on Sephadex LH-20,Huthig Cerlag Heidelgerg, 1995:196 Preparative Gel Chromatography on Sephadex LH-20,Huthig Cerlag Heidelgerg, 1995:198 Preparative Gel Chromatography on Sephadex LH-20,Huthig Cerlag Heidelgerg, 1995:208 1999 34 9 10 2000 12 1
7

YMC
YMC
YMC
YMC Waters YMC 40-60 15 m 20 m 10 m m 50 150 m ODS YMC m m
ODS-A
ODS
C18
50 m 75 m 150
m 75
Column : YMC Pack ODS-AQ (S-50
m ,12nm (120A) )
Eluent : methanol/water (85/15) Detection : UV at 254 nm , 0.16AUFS Sample : 1. Methyl benzoate 2. Toluene
ODS-50um/
ODS-40~60m/ Eluent : methanol/water (85/15) Detection : UV at 254 nm , 0.32AUFS 3. Toluene 4. Naphthalene
ODS-AQ
YMC-Pack ODS-AQ YMC-Pack ODS-A YMC-Pack ODS-AQ
C18
Column size : 500 x 20 mmI.D. Flow rate : 40mL/min Sample : 1. Uracul Injection : 100 L 2. Methyl benzoate
5
m,10
m,50
m
ODS-A S-10 N=9000 P=3.4MPa m 12nm ODS-A S-50 N=1000 P=0.3MPa m 12nm
YMC
ODS-A S-5 N=25000 P=19.6MPa
m 12nm
AQ
ODS-AQ
, ODS
,
8

YMC
YMC
A 10 120 ODS-A 15 20 50 10 15 10 15 10 15 20 50 10 15 10 15 10 50 10 15 10 10 10 15 10 15 10 15 20 10 15 20 50 10 15 10 15 m AA12S11 AA12S16 AA12S21 AA12S50 AA20S11 AA20S16 AA30S11 AA30S16 AQ12S11 AQ12S16 AQ12S21 AQ12S50 AQ20S11 AQ20S16 AS12S11 AS12S16 PC99S10 PC99S50 OC12S11 OC12S16 OC20S11 BU12S11 BU20S11 BU20S16 BU30S11 BU30S16 SL06S11 SL06S16 SL06S21 SL12S11 SL12S16 SL12S21 SL12S50 SL20S11 SL20S16 SL30S11 SL30S16 pH PTC-
C18
200 300
100% ODS-AQ
120
200 Pro C18 Polymer C18 C18 999% pH C8 120 200 120 C4 C4 200 300 99. 120
C8
60
Silica
120
200 300
9

Fuji
Fuji
70
Chromatorex
Chromatorex A m
R
R
SPS, SMB, MB,GS
100 200 300 2 100A 3 5 C18, C8, C4, Diol 10 NH 2 HPLC HPLC
SPS
Chromatorex R SMB A m
70 100 150 200 300 500 3 5 10 15 20 20 - 45 C18, C8, C4, Diol NH 2
800
1000
HPLC, MPLC
Chromatorex R MB A m
70 100 300 500 40-75 75-200 C18, C8, C4, Diol
800 NH 2
1000
Flash
Chromatorex R GS A m
60 20- 45 40- 75 75- 200 C18, C8, NH 2 ( )
10

Daiso
Daiso
DAISOGEL ODS-AP
ISO900 AP DAISOGEL ODS-AP ODS-AP ODS-BP 100% ODS-BIO (HAS) ODS-P C8-P C4-BIO C4-P C1-p APS-P SP-P 60A 100A 120A 200A 200A 300A DAISOGEL ODS-
C18 C4
Amino-Propyl
DAISOGEL ODS-BP
C18 DAISOGEL ODS-BP
DAISOGEL ODS-BP
PH
LC-MS
SP-P
SP-P 10ppm, 300 A 7 60 m 10 m m 15 3 m 4 m m 20 Al Fe Ti Zr 60 A 120 A 200A m 5 m 40-60 m 40-
010-******** https://www.360docs.net/doc/9d9111799.html, .
DSISO
11

Merck
Merck
TLC HPLC LC
1.10180.1000 1.10181.1000 1.15111.1000 1.09385.1000 1.07729.1000 1.15101.1000 1.07734.1000 1.07754.0500 1.07733.0500 1.10184.0500 1.10185.0500 1.10757.1000 1.07719.0250 1.10167.1000 1.10601.0500 1.02693.0250 1.02331.0500 1.03201.0250 1.12518.0100 1.01067.1000 1.01076.1000 1.01077.1000 1.01078.1000 1.01097.1000 1.01061.1000 1.09319.0100 1.09336.1000 1.09390.1000 1.13905.0250 1.13904.1000 1.09304.0100 1.09348.0100 1.13903.0500 1.09324.0100 1.09362.0250 1.13902.0100 1.13901.0500 1.09303.0100 1.13900.0250 1.13973.0250 1.13925.0100 1.13974.0250 1.13959.0250 1.19654.0100 1.11024.0100 1.19656.0100 1.11022.0100 1.19655.0100 1.11023.0100 1.19662.0100 1.19659.0100 1.50075.0010 1.16362.0050
1kg 1kg 1kg 1kg 1kg 1kg 1kg 0.5kg 0.5kg 0.5kg 0.5kg 0.5kg 0.25kg 1kg 0.5kg 0.25kg 0.5kg 0.25kg 0.1kg 1kg 1kg 1kg 1kg 1kg 1kg 0.1kg 1kg 1kg 0.25kg 1kg 0.1kg 0.1kg 0.5kg 0.1kg 0.25kg 0.1kg 0.5kg 0.1kg 0.1kg 0.1kg 0.1kg 0.25kg 0.25kg 0.1kg 0.1kg 0.1kg 0.1kg 0.1kg 0.1kg 0.1kg 0.1kg 10g 50g
Silica gel 40,63-200 m Silica gel 40,200-500 m Silica gel 60,15-40 Silica gel 60,40-63 m m
40 40 60 60 60 60 60 60 60 100 100 60 60, C18
40 A 63-200 m 40A 200-500 m 60 A 15-40 m 60 A 40-63 m 60 A <63 m 60 A 63-100 60 A 63-200 60 A m m
Silica gel 60,<63 m Silica gel 60,63-100 m Silica gel 60,63-200 Silica gel 60 Silica gel 60,200-500 Silica gel 100,63-200 m m m
60A ,200-500 m 100A,63-200 m 100A,200-500 m 60 A ,63-200 m m
Silica gel 100,200-500 m Silica gel 60F254,63-200 m Silica gel 60 silanized,63-200 m Silica gel 60,RP-18 40-63 m Kieselguhr Celite 545 Cellulose microcrystalline DEAE-cellulose Florisil ,150-250 m Aluminium Oxide 60 Aluminium Oxide 90 63-200 Aluminium Oxide 90 63-200 Aluminium Oxide 90 63-200 m m m T
100 A ,63-200 m 60 A 40-63
150-250 60 90 90 90 90 60 60 60 60 100 C2 C8 C8 C8 C8 C18 C18 C18 C18
m 90 A 90 A 90 A 63-200 63-200 63-200 m m m
Aluminium Oxide 90 63-200 m Aluminium Oxide 150 63-200 m Lichroprep Si60,5-20 m Lichroprep Si60,15-25 m Lichroprep Si60,25-40 Lichroprep Si60,40-63 Lichroprep Si100,25-40 Lichroprep RP-2,25-40 m m m m
90 A 63-200 m 15 A 63-200 m 60 A 60 A 60 A 60 A 5-20 m 15-25 m 25-40 40-63 m m m
100 A 25-40 m 100 A 25-40 100 A 100 A 100 A 100 A
Lichroprep RP-8,5-20 m Lichroprep RP-8,15-25 m Lichroprep RP-8,25-40 Lichroprep RP-8,40-63 m m
5-20 m 15-25 m 25-40 40-63 m m
Lichroprep RP-18,5-20 m Lichroprep RP-18,15-25 m Lichroprep RP-18,25-40 Lichroprep RP-18,40-63 Lichroprep DIOL,40-63 Lichroprep NH2,25-40 m m m m
100 A 5-20 m 100 A 15-25 m 100 A 25-40 m 100 A 40-63 m 100 A 100 A 100 A 60 60 100 A 25-40 40-63 40-63 60 A 60 A 12 15 12 15 100A 100A 300 A 300 A 12 15 40-63 m m m m m m m 12 15 m m m m m
Lichroprep NH2,40-63 m Lichroprep CN,40-63 m Lichrospher Si60,12 Lichrospher Si60,15 m m m m m m m m C18 C18
Lichrospher 100RP-18,12 Lichrospher 100RP-18,15
100 A 100 A
Lichrospher 60RP-SelectB,12 Lichrospher 60RP-SelectB,15 Lichrospher WP300RP-18,12 Lichrospher WP300RP-18,15 ChiraSpher Cellulose triacetate
12

Merck
Merck
1989
Fractogel EMD
MERCK
Fractogel EMD Fractogel EMD 5 Fractogel Fractogel EMD Fractogel EMD 20~40 20~40 m 40~90 m
m 800A
1.16881.0500 1.16887.0100 1.16887.0500 1.10316.5000 1.16883.0500 1.16888.0010 1.16888.0500 1.16884.0500 1.16889.0010 1.16889.0500 1.16882.0500 1.16890.0010 1.16890.0500 1.14894.0500 1.16886.0500 1.16891.0010 1.16891.0500 1.10085.0010 1.10085.0500 1.16197.0010 1.16197.0500 1.10338.0250 1.16426.0025 1.16473.0025 1.14893.0500 1.16961.0100 1.12930.0010 1.10087.0010 1.10317.0150
500ml 100ml 500ml 500ml 500ml 100ml 500ml 500ml 100ml 500ml 500ml 100ml 500ml 500ml 500ml 100ml 500ml 100ml 500ml 100ml 500ml 250ml 25ml 25ml 500ml 100g 10g 10g 150ml
Fractogel¨EMD TMAE 650(M), Fractogel¨EMD TMAE 650(S), Fractogel¨EMD TMAE 650(S), Fractogel¨EMD TMAE Hicap(M), Fractogel¨EMD TMAE 650(M), Fractogel¨EMD TMAE 650(S), Fractogel¨EMD TMAE 650(S), Fractogel¨EMD TMAE 650(M), Fractogel¨EMD TMAE 650(S), Fractogel¨EMD TMAE 650(S), Fractogel¨EMD S03- 650(M), Fractogel¨EMD S03- 650(S), Fractogel¨EMD S03- 650(S), Fractogel¨EMD SE Hicap(M), Fractogel¨EMD COO-650(M), Fractogel¨EMD COO-650(S), Fractogel¨EMD COO-650(S), Fractogel¨EMD propyl 650(S), Fractogel¨EMD propyl 650(S), Fractogel¨EMD Phenyl 650(S), Fractogel¨EMD Phenyl 650(S), Fractogel¨EMD Chelate 650(M), Fractogel¨EMD Chelate 650(S), Fractogel¨EMD TA 650(S), Fractogel¨EMD Amino 650(M), Fractogel¨EMD Epoxy 650(M), Fractogel¨EMD Heparin 650(S), Fractogel¨EMD Azlactone 650(M), Fractogel¨EMD BioSEC(S), 20-40 20-40 40-90
40-90 20-40 20-40 40-90 20-40 20-40 40-90 20-40 20-40 40-90 20-40 20-40 40-90 20-40
m m m 40-90 m m m m m m m m m 40-90 m m m m
20-40 m 20-40 m 20-40 20-40 20-40 m m m 40-90 20-40 m m 40-90 m 20-40 m 20-40 m m m m
13

AMBERCHROM
AMBERCHROMTM
(1) CG AMBERCHROMTM CG 1-14 AMBERCHROMTM CG pH
Sillca
Water Methanol Ethanol Isopropanol Acetonitrile Acetone Toluene Dry
100 108 106 112 112 114 104 90
CG161 CG300 CG1000 CG71
m2/g 900 700 200 500
A 150 300 1000 250
m 35 70 120 35 70 120 35 35 70 120
D90/D40 1.7 1.7 1.7 1.7
AMBERCHROMTM CG 161 AMBERCHROMTM CG 161 AMBERCHROMTM CG 161
Amberchrom CG161
AMBERCHROMTM CG161
1. 6-AMINOPENICILLANIC ACID 2. PENICILLIN G 3. PENCILLIN V COLUMN: SOLVENT GRADIENT: 6.3 X 1.0 DIAMETER A: 50mMPHOSPHATE, pH 8.0 B: METHANOL 4 MIN ISOCRATIC 60% B
25 MIN TO 100% B FLOW RATE: 1.0 ML/MIN
14

AMBERCHROM
CG161M
/ Polyphenolics /Flavanoids /Saponins
AmberliteTM XADTM 7HP AmberliteTM XADTM 16HP AmberliteTM XADTM 7HP AmberliteTM XADTM 16HP AmberliteTM XADTM 16HP AmberliteTM XADTM 7HP AmberliteTM XADTM 7HP AmberliteTM XADTM 16HP AmberliteTM XADTM 16HP
AmberchromTM CG 71 AmberchromTM CG161 AmberchromTM CG71 AmberchromTM CG161 AmberchromTM CG300 AmberchromTM CG161 AmberchromTM CG300 AmberchromTM CG71 AmberchromTM CG71 AmberchromTM CG161 AmberchromTM CG300 AmberchromTM CG161 AmberchromTM CG161 AmberchromTM CG300 AmberchromTM CG161 AmberchromTM CG161 AmberchromTM CG300
/Anthocyanin / Notoginsenosides / Colchicine / Taxol E/ Tocopherol Isoquinoline (alkaloid) / Ellagitannins Prickly Ash Bark
AmberliteTM XADTM 16HP AmberliteTM XADTM 16HP AmberliteTM XADTM 16HP
(2) XT
Sillca AMBERCHROM
TM
XT
AMBERCHROMTM XT
20
30
Conditions Column: Flow rate Detritylation: Elution: Mobile Phase
AMBERCHROM XT20, 1cm I.D. x 25cm L Loading and Equilibration: 4 mL/minute 4 mL/minute 1 mL/minute A: 0.1M TEAA, pH 7.0 B: 20% acetonitrile in 0.1M TEAA, pH 7.0 C: 0.5% TFA (for detritylation)
50bars Sillca
Gradient Sample
20% - 55% B in 10 column volumes (200mL) 20-mer phosphodiester oligonucleotide (5 -AGC TAG CTA GCT AGC TAG CT-3 ) DMT-on, not fully deprotected
A AMBERCHROM XT20 AMBERCHROM XT30 300 300 20 30
m 60 bars 60 bars
pH 1-14 1-14 4-60 4-60
15

MCI GEL
MCI GEL
MCI GEL Diaion Sepabeads HPLC MCI GEL 4 pH m 300 m
1-12
MCI GEL
MCI GEL
MCI GEL
MCI GEL CHP10M MCI GEL CHP 5C MCI GEL CHP 55A MCI GEL CHP 55Y MCI GEL CHP 20Y MCI GEL CHP20P MCI GEL CHP 20SS MCI GEL CHP 2MG M MCI GEL CHP 2MG MCI GEL CHP 2MG Y * MCI GEL CHP 20P 75~150 m 37~75
4 9~11 15~20 25~35 25~35
m m m m m m
4 10 18 30 30 55 100 4 10 31
m m m m m m m m m m
640m2/g 540m2/g 580 m2/g 590 m2/g 560 m2/g 520 m2/g 540 m2/g 460 m2/g 590 m2/g 510 m2/g
1.45ml/g 1.39ml/g 1.54 ml/g 1.55 ml/g 1.67 ml/g 1.17 ml/g 1.35 ml/g 1.09 ml/g 1.13 ml/g 1.15 ml/g
14.0nm 14.0nm 14.0 nm 14.0 nm 22.0 nm 30.0 nm 29.0 nm 27.0 nm 20.0 nm 23.0 nm
m 75~150 63~150 m m 4 9~11 m 25~35 m
16

Hisep Hisep
1 2 1M NaOH 3 4 pH 1M HCl
1 2 3
mm HE-RP07M1546C HE-RP10M1546C HE-RP20M1546C HE-RP07H1546C HE-RP10H1546C HE-RP20H1546C HE-RP07H2546C HE-RP10H2546C HE-RP20H2546C 150 4.6 150 4.6 7 10 20 7 10 20 7 10 20
m
Mpa 30 10 10 30 10 10 30 10 10
pH( 2
/ 13/1 14
)
2
13/1
14
250
4.6
2
13/1
14
HE-RP10M2510C HE-RP10H2510C HE-RP10M2520C HE-RP10H2520C HE-RP10M1520C HE-RP10H1520C HE-RP20M2550C HE-RP30M2510C
250 250 250 250 150 150 250 250
mm 10 10 20 20 20 20 50 100
m 10 10 10 10 10 10 20 30
Mpa 10 10 10 10 10 10 10 10
pH( / ) 2 13/1 14 2 2 2 2 2 2 2 13/1 13/1 13/1 13/1 13/1 13/1 13/1 14 14 14 14 14 14 14
m HE-RP10L10P HE-RP10L50P HE-RP20L10P HE-RP20L50P HE-RP50L10P HE-RP50L50P HE-RP10M10P HE-RP10M50P HE-RP20M10P HE-RP20M50P HE-RP50M10P HE-RP50M50P HE-RP10MH10P HE-RP10MH50P HE-RP20MH10P HE-RP20MH50P HE-RP50MH10P HE-RP50MH50P HE-RP10H10P HE-RP10H50P HE-RP20H10P HE-RP20H50P HE-RP50H10P HE-RP50H50P 10 20 50 10 20 50 10 20 50 10 20 50 10 50 10 50 10 50 10 50 10 50 10 50 10 50 10 50 10 50 10 50 10 50 10 50
mL)
pH( / ) 2 13/1 14 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 13/1 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14
20
m
50
m
17

GE Healthcare SEC
GE Healthcare SEC
]Superdex
0.1M HCI FPLC HPLC
1M NaOH 40 400h Tricorn HR XK Superdex
( m)
BPG 10/300GL
/ pH MPa cm/h
17-0905-10 17-0905-01 17-0905-03 17-0905-04 17-1044-10 17-1044-01 17-1044-02 17-1044-04 17-1043-10 17-1043-01 17-1043-02 17-1043-01 Superdex200 prep grade Superdex75 prep grade Superdex30 prep grade
25ml 150ml 1L 5L 25ml 150ml 1L 5L 25ml 150ml 1L 5L 10,000600,000 22-44 3-12[1-14] 0.3 90 3000-70,000 22-44 3-12[1-14] 0.3 90 <10,000 22-44 3-12[1-14] 0.3 90
]Superose 8M
( 17-0489-01 17-0489-03 17-0489-04 17-0536-01 17-0536-03 17-0536-04 Superdex6 prep grade Superdex12 prep grade 125ml 1L 5L 125ml 1L 5L 5000-5x106
m)
/
pH MPa 3-12[1-14] 0.4 cm/h 40
20-40
1000-300,000
20-40
3-12[1-14]
0.7
40
]Sephacryl
0.5M NaOH HiPrep 16 26/60 Sephacryl 100 200 300HR
( 17-0612-01 17-0612-05 17-0612-10 17-0584-01 17-0584-05 17-0584-10 17-0599-01 17-0599-05 17-0599-10 Sephacryl S-100 HR Sephacryl S-200 HR Sephacryl S-300 HR 750ml 10L 150ml 750ml 10L 150ml 750ml 10L 150ml 5000-250,000 25-75 (lgG/MAB) 3-11[2-13] 0.2 60 100-100,000 m) / pH MPa 3-11[2-13] 0.2 cm/h 60
25-75
10,000-1.5*106
25-75
(lgG/MAB)
3-11[2-13]
0.2
60
18

GE Healthcare SEC
17-0609-01 17-0609-05 17-0609-10 17-0613-01 17-0613-05 17-0613-10 17-0476-01 17-0476-05 Sephacryl S-400 HR Sephacryl S-500 HR Sephacryl S-1000 SF 750ml 10L 150ml 750ml 10L 150ml 750ml 10L
20,000-8x106
25-75
<25,000bpDNA
3-11[2-13]
0.2
60
40,0002x107 5x10 5 1108
25-75
<25,000bpDNA DNA
3-11[2-13]
0.2
50
40-105
<300-400nm
3-11[2-13]
40
]Sepharose Fast Flow
1-2M NaOH
( 17-0159-01 17-0159-05 17-0149-01 17-0149-05 Sepharose6 Fast Flow Sepharose4 Fast Flow 1L 10L 1L 10L <10,0004x106 60,00020x106 m) / DNA pH MPa 2-12[2-14] 2-12[2-14] 0.1 0.1 cm/h 300 250
45-165 45-165
] Sepharose 10,000 40,000,000
( 17-0130-01 17-0130-05 17-0120-01 17-0120-05 17-0110-01 17-0110-05 Sepharose 2B Sepharose 4B Sepharose 6B 1L 10L 1L 10L 1L 10L 70,000 -4x106 60,000 -20x106 10,000 -4x106 m) / pH MPa [ ] 4-9[3-11] 0.004 cm/h 10
60-200
45-165 45-165
4-9[3-11] 4-9[3-11]
0.008 0.02
11.5 14
] Sepharose CL Sepharose 2,3 Sepharose
( 17-0140-01 17-0140-05 17-0150-01 17-0150-05 17-0160-01 17-0160-05 Sepharose CL-2B Sepharose CL-4B Sepharose CL-6B 1L 10L 1L 10L 1L 10L 70,000 -40x106 60,000 -20x106 10,000 -4x106 m) / pH MPa 3-13[2-14] / 45-165 45-65 / / 3-13[2-14] 3-13[2-14] 0.0012 0.02 26 30 0.005 cm/h 15
Sepharose
60-200
19

GE Healthcare SEC
] Sephadex [epicnlorohydrin] Coarse Bioprocess
( 17-5064-01 17-5064-02 17-0020-01 17-0020-02 17-0020-03 17-0034-01 17-0034-02 17-0034-03 17-0033-10 17-0033-01 17-0033-02 17-0033-03 17-0032-01 17-0032-02 17-0032-03 17-0031-01 17-0031-02 17-0031-03 17-0044-01 17-0044-02 17-0044-03 17-0043-01 17-0043-02 17-0043-03 17-0042-01 17-0042-02 17-0042-03 17-0041-01 17-0041-03 17-0050-01 17-0050-02 17-0050-03 17-0050-01 17-0051-03 17-0060-01 17-0060-02 17-0060-03 17-0060-15 Sephadex 17-0061-01 G-100 Superfine Sephadex G-150 100g 2000-9x104 20-50 2-10[2-13] 12 Sephadex G-100 Pharmadex G-10 Sephadex G-15 Sephadex G-25Coarse Sephadex G-25 Medium Sephadex G-25Fine Sephadex G-25 Superfine Sephadex G-50Coarse Sephadex G-50 Medium Sephadex G-50 Fine Sephadex G-50 Superfine Sephadex G-75 Sephadex G-75 Superfine 100g <700 500g 100g 500g 5kg 100g 500g 5kg 25g 100g 500g 5kg 100g 500g 5kg 100g 500g 5kg 100g 500g 5kg 100g 500g 5kg 100g 500g 5kg 100g 1000-30000 5k 100g 500g 5kg 100g 2000-70000 5kg 100g 500g 5kg 1.5kg 2000-12x104 40-120 2-10[2-13] 50 20-50 ( ) 2-10[2-13] 18 3000-0000 40-120 2-10[2-13] 77 20-50 2-10[2-13] 1000-30000 20-80 2-10[2-13] 60 1000-30000 100-300 2-10[2-13] 1000-5000 20-80 2-13[2-13] 60 1000-5000 50-150 2-13[2-13] 150 100-1500( ) 40-120 2-13[2-13] 40-120 2-13[2-13] 40 m) / pH MPa cm/h
Fine
Sephadex
1000-5000( )
100-300
2-13[2-13]
-
1000-5000
20-50
2-13[2-13]
20
1000-30000
50-150
2-10[2-13]
-
17-0070-02
500g
5000-3x105
40-120
2-10[2-13]
23
]
17-0441-01 17-0441-02
Marker
Calibration Kit HMW For GEL FILTRation Calibration Kit LMW For GEL FILTRation 17-0360-01 158000 13700 Blue Dextran 2000 232000 25000 440000 669000 2000000 500g 50mg
43000 67000 2000000
2000000
20